BIOL 101 Kowalko mkcullen Transcription and Translation
Transcription
and
Translation
1. Describe
the
general
layout
of
the
Central
Dogma,
draw
a
"flow
chart"
to
help
you
see
the
order
of
the
steps,
and
include
which
transition
of
steps
are
transcription
and
translation.
DNA?RNA?polypeptide.
DNA
to
RNA
is
transcription
and
RNA
to
polypeptide
is
translation
2.
(RNA/DNA)
is
used
to
make
an
order
of
amino
acids
in
a
protein
3. What
is
a
codon?
What
does
it
code
for?
Draw
what
it
looks
like.
A
triplet
of
nucleotides
(A,
T,
G,
C)
codes
for
amino
acid.
4. What
does
"the
reading
frame"
refer
to?
A
bunch
or
triplet
of
3
nucleotides
is
how
you
would
read
them
(3
at
a
time)
5. You
have
a
strand
of
DNA
that
is
the
following:
TACGATAGG.
Find
the
amino
acids
that
would
be
coded
from
this
example.
BE
CAREFUL!
Remember
the
steps
of
the
Central
Dogma
AUGCUAUCC
(RNA
STRAND)
AUG:
Met
CUA:
leu
UCC:
ser
6. In
Eukaryotes,
what
do
the
steps
of
Transcription
look
like?
(you
can
draw
another
flow
chart
if
it
helps
you)
DNA?mRNA?RNA?protein
(polypeptide)
7. Describe
generally
what
happens
in
initiation,
elongation,
and
termination.
Initiation:
Start
at
the
promoter
(start
point:
TATA
box)
RNA
polymerase
unwinds
strand
of
DNA
Elongation:
RNA
polymerase
adds
the
mRNA
to
the
template
strand
of
DNA
Termination:
DNA
winds
back
together,
and
RNA
polymerase
will
reach
a
stopping
point
(codon
to
terminate
or
stop
transcription)
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