DNA replication
DNA replication:
? Copying genetic information for transmission to the next generation
? Occurs in S phase of cell cycle
? Process of DNA duplicating itself
? Begins with the unwinding of the double helix to expose the bases in each strand of DNA
? Each unpaired nucleotide will attract a complementary nucleotide from the medium
? will form base pairing via hydrogen bonding.
? Enzymes link the aligned nucleotides by phosphodiester bonds to form a continuous strand.
1
DNA replication:
? First question asked was whether duplication was semiconservative or conservative
? Meselson and Stahl expt
? Semiconservative -
? one strand from parent in each new strand
? Conservative-
? both strands from parent and other is all new strands
2
DNA replication:
? Complementary base pairing produces semiconservative replication
? Double helix unwinds ? Each strand acts as template ? Complementary base pairing ensures that T signals
addition of A on new strand, and G signals addition of C ? Two daughter helices produced after replication
3
4
Experimental proof of semiconservative replication ? three possible models
? Semiconservative replication ?
? Watson and Crick model
? Conservative replication:
? The parental double helix remains intact; ? both strands of the daughter double helix are newly
synthesized
? Dispersive replication:
? At completion, both strands of both double helices contain both original and newly synthesized material.
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