SoftMax Pro User’s Manual - Boyce Thompson Institute

SoftMax? Pro User's Manual

Version 4.3 Software for Macintosh? and Windows?

Molecular Devices Corporation

Sunnyvale, California 94089

Part # 5900-0328 Rev. A

Molecular Devices Corporation SoftMax? Pro User's Manual

COPYRIGHT ? Copyright 2002, Molecular Devices Corporation. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language or computer language, in any form or by any means, electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without the prior written permission of Molecular Devices Corporation.

TRADEMARKS AND PATENTS FlexStation, FLIPR, SoftMax, SpectraMax, and Vmax are registered trademarks and Automix, Emax, PathCheck, ThermoMax, UVmax, and VersaMax are trademarks of Molecular Devices Corporation. All other trademarks or registered trademarks are the property of their respective owners.

Automix is covered by U.S. Patent Number 5,112,134; PathCheck is covered by U.S. Patent Number 5,959,738. These trademarks are not to be used in any type of promotion or advertising without written permission from Molecular Devices Corporation.

DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. This manual is subject to change without notice. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices Corporation assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. This manual does not represent a commitment on the part of Molecular Devices Corporation. The software described in this document is furnished under a license agreement or nondisclosure agreement. The software may be used or copied only in accordance with the terms of the agreement. It is against the law to copy the software on any medium except as specifically allowed in the license or nondisclosure agreement.

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SOFTmax PRO User's Manual

SOFTMAX PRO LICENSE AGREEMENT AND WARRANTY

Important: Please read this license agreement carefully before opening the software package. The right to use this Molecular Devices Corporation ("MDC") software is sold only on the condition that the user agrees to the terms of this license agreement. If you do not agree to the terms of this license, you may return the unopened package to MDC or its authorized agent for a full refund. However, opening the software package indicates your acceptance of these terms and conditions.

LICENSE AGREEMENT

In consideration of payment of the license fee, which is part of the purchase price you paid for this product, Molecular Devices Corporation ("MDC"), the licensor, hereby grants a non-exclusive license to you, the Licensee ("User"), to use SoftMax Pro and it's utilities ("Software") included with SoftMax Pro upon license. The user is licensed to copy the software for a backup or archival copy as necessary. Simultaneous use of the software with more than one MDC Microplate Reader System per license is expressly prohibited. Simultaneous use of the software on four (4) computers solely for the purpose of analyzing already acquired data is expressly permitted. The user is expressly prohibited from using the software to control any other instrument, computing hardware, or peripherals other than MDC Microplate Systems.

This license agreement does not grant the user title to the software, or any copyrights of proprietary rights in the software. The user may not sublicense, rent, lease, modify, translate, decompile, or disassemble the software for any purpose. The User, however, does own the media on which the software is originally or subsequently recorder or fixed. MDC retains title and ownership of the software recorded on the original disc and all subsequent copies of the software, regardless of the form or media in or on which the original and other copies may exist. This license is not a sale of the original software or any copy.

LIMITED WARRANTY

MDC warrants that the disc on which the program is furnished is free of defects in materials and workmanship and will operate under normal use for a period of 90 days from the date of delivery. Except as provided above, the Software is provided "as is" without additional warranties of any kind either express or implied, including, but not limited to, any implied warranty of merchantability or fitness for a particular purpose. The entire risk as to the quality and performance of the Software is the User's. MDC does not warrant that the functions contained in the Software will meet the User's requirements nor that the operation of the program will be uninterrupted or error free. Some states do not allow the exclusion of implied warranties and, therefore the above exclusion may not apply to you.

LIMITATION OF REMEDIES

MDC's entire liability and the User's only remedy with respect to the Limited Warranty set forth above is:

1) The replacement of any discs not meeting the Limited Warranty set forth above, provided such discs are returned to MDC with evidence of date of purchase; or

2) If MDC is unable to deliver a replacement disc which is free of defects in materials and workmanship, the user may terminate this license agreement upon return of all materials including manual and disc(s) to MDC for a full refund of the purchase price.

In no event will MDC be liable to the User for any damages, including any lost profits, lost savings, or other incidental or consequential damages arising out of the use or inability to use the software even if MDC has been advised of the possibility of such damages, or for any claim by another party. Some states do not allow the limitation or exclusion of liability for incidental or consequential damages so the above limitations or exclusions may not apply to you.

SOFTmax PRO User's Manual

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CLARIFICATION

Life Science Edition

SoftMax Pro for Life Sciences is licenced to one MDC microplate reader system. Simultaneous use of the software with more than one microplate reader is expressly prohibited. Simultaneous use of the software on three (3) additional computers (in addition to the computer controlling the instrument, if applicable) solely for the purpose of analyzing previously-acquired data is expressly permited.

Drug Discovery Edition

SoftMax Pro for Drug Discovery is licenced to one MDC microplate reader system. Simultaneous use of the software with more than one microplate reader is expressly prohibited. Simultaneous use of the software on three (3) additional computers (in addition to the computer controlling the instrument, if applicable) solely for the purpose of analyzing previously-acquired data is expressly permited.

Enterprise Edition

SoftMax Pro Enterprise is sold as a site license. A license for each reader-connected computer and analysisseat computer needs to be purchased.

Additional software licenses can be purchased by calling our Customer Service department at (800) 6355577

DURATION AND TERMINATION

The License is effective from the date of the first use of the Software by the User until terminated or until the expiration of fifty years, whichever comes first. The User may terminate the License by returning to MDC all Software documentation, the original Software, all backup and archival copies of the Software, and all demonstration copies of the Software. The License agreement will terminate automatically without notice from MDC if the User fails to comply with any provision of this License Agreement. It is incumbent upon the User to take all reasonable measures to effect termination only in accordance with this License Agreement.

GENERAL

1) This License Agreement shall be governed by the laws of the State of California.

2) The provisions of this License Agreement are severable and if any one or more of such provisions are judicially determined to be illegal or otherwise unenforceable, whether in whole or in part, those remaining provisions or portions of the License Agreement unaffected by such determination shall be binding on and enforceable by and between the User and MDC.

3) In the event it becomes necessary to retain the services of an attorney to enforce any provisions of this License Agreement, the non-prevailing party to such legal action and/or litigation agrees to pay the prevailing party's costs, including reasonable attorney's fees and court costs, if any.

4) The User acknowledges that by opening the media package the User has read this License Agreement, understands it, and agrees to be bound by its terms and conditions. The User further agrees that this License Agreement constitutes the full and complete agreement between the User and MDC with respect to the Software and the License, which agreement supersedes any proposal or prior agreement, whether oral or written, and any other communication between the User and MDC relating to this License Agreement.

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SOFTmax PRO User's Manual

Contents

Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-xi Tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-xix

Chapter 1: Welcome to SoftMax Pro. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3 New to SoftMax Pro? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4 What's New in SoftMax Pro Version 4.3 . . . . . . . . . . . . . . . . . . . . . . . . .1-4 About the Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6 Conventions Used in This Manual. . . . . . . . . . . . . . . . . . . . . . . . .1-7 Getting Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9 Macintosh On-Line Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9 Windows On-Line Help. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10 Troubleshooting and Technical Support. . . . . . . . . . . . . . . . . . .1-12

Chapter 2: Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1 Connect the Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3 Connecting to a Macintosh . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3 Computer System Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6 Macintosh Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6 Windows-Compatible Computer. . . . . . . . . . . . . . . . . . . . . . . . . .2-7 Install SoftMax Pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8 What Is Installed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-9 Enter Registration Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-13 Communicating with the MDC Microplate System Instrument . . . .2-15 Set Preferences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-16 Serial Port. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-16 Export Format . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-17 Reader. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18 Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-19 Autosave . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-20 Autoprint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-22 Assays Folder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-23

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