F actin antibodies

• [DOCX File]Genes & Development

  https://info.5y1.org/f-actin-antibodies_1_c1f775.html

  Maximum intensity projection of a confocal z-stack showing F-actin in red, acetylated tubulin in green and DAPI in blue. Note that the actin cytoskeleton (and apical actin microvilli) distribution does not change upon depletion of CEP83 (right) compared to control (left). Table S1: Evolutionary profile of novel DAP proteins in ciliated organisms

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• [DOC File]Introduction - Ohio University

  https://info.5y1.org/f-actin-antibodies_1_e04368.html

  The following antibodies/fluorescent probes should stain the following organelles/structures: Mouse monoclonal E7 anti-β-tubulin (DSHB): microtubules. Use Goat anti-mouse IgG Alexa-488 secondary (green) antibody. ... Phalloidin: a mushroom toxin that binds avidly to F-actin.

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• [DOC File]Cell

  https://info.5y1.org/f-actin-antibodies_1_9038f7.html

  The amount of actin recovered in last extraction was negligible (less than 5% of total actin), ascertaining almost complete recovery of actin. 10 (l of supernatants from the G- and F-actin fractions were loaded onto 15% SDS-PAGE and analyzed by immunoblot using anti-actin (chicken) antibody.

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• [DOC File]WordPress.com

  https://info.5y1.org/f-actin-antibodies_1_626b87.html

  Cells were then probed with rhodamine phalloidin to visualize F-actin and anti-phosphotyrosine followed by FITC-conjugated secondary antibody to visualize focal adhesions, as described in Ref. [45]. Micrographs at x600 magnification were digitally captured using a SpotII digital camera and software (Diagnostic Instruments, Inc., Sterling ...

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• Table S2: Cytoplasmic HEp-2 patterns (further details)

  Seret G, Canas F, Pougnet-Di Costanzo L, et al. Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis. J Autoimmun 2015;61:54-61.

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• [DOC File]www.researchgate.net

  https://info.5y1.org/f-actin-antibodies_1_61a2ee.html

  In dendrites of DIV5 hippocampal neurons, clear colocalization is detected between transfected BAI3 (BAI3) and F-actin (stained with phalloidin-TRITC), in particular in protrusions (arrows). Scale ...

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• [DOCX File]Supporting Online Material for - Cancer Discovery

  https://info.5y1.org/f-actin-antibodies_1_02486c.html

  Membranes were incubated in the following primary antibodies (1:1000) overnight: PD-L1 (CST), total and phospho- (S366) STING (CST), total and phospho- (S172) TBK1 (CST), total and phospho- (S396) IRF3 (CST), cGAS (CST), Histone H3, pro and cleaved caspase 3, pro and cleaved caspase 9, Lamin B1 and Actin. Secondary antibodies . were purchased ...

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• [DOCX File]files.emailmeform.com

  https://info.5y1.org/f-actin-antibodies_1_6e0fbe.html

  In the first two experiments, phalloidin was used specifically to stain F-actin based stereocilia, while acetylated tubulin was used to bind to microtubule based kinocilia. In the third experiment, acetylated tubulin and PH3B were used as primaries; acetylated tubulin was used to ... Secondary antibodies used included Goat anti rabbit alexa ...

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• [DOCX File]Genes & Development

  https://info.5y1.org/f-actin-antibodies_1_1c4dcc.html

  Cells were then incubated consecutively with primary antibodies (overnight at 4°C) and Alexa Fluor 488-labeled goat secondary antibodies (for 90 min at room temperature) in PBS containing 1% bovine serum albumin (BSA). For staining of F-actin, cells were incubated with 6.6 nM Alexa Fluor 488-phalloidin for 90 min after blocking.

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• [DOC File]Immunofluorescence of Endothelial Cells

  https://info.5y1.org/f-actin-antibodies_1_41c4e7.html

  **If staining for F-actin, use 1U/coverslip of Rhodamine-Phalloidin (Molecular Probes). Prepare solution by diluting stock 200 Unit/ml solution 1:50 in blocking solution (add 1.5 ml blocking solution in 30 ul stock R-P). If double labeling, primary antibody can be diluted in the R-P solution.

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• www.cell.com

  mutant exposed to continuous blue light (20 μmol/m2/s) for different time were subjected to immunoblot analysis with anti-HY5 and anti-ACTIN antibodies, respectively. (B) Yeast two-hybrid plate assay showing no interaction between AGB1 and COP1. BD-AGB1/AD-CRY2 served as positive control.

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• [DOC File]INTRODUCTION - Susquehanna University

  https://info.5y1.org/f-actin-antibodies_1_3643ea.html

  You will treat the cover slips with the secondary (2°) antibody - a TRITC-conjugated anti-mouse antibody. The 2° ab staining solution also contains FITC -phalloidin, which will bind to and stain F-actin green, and DAPI to stain the DNA in the cell blue. Finally, the cover slips will be mounted with Prolong Antifade mounting solution. 1.

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• [DOCX File]TF_Template_Word_Windows_2016

  https://info.5y1.org/f-actin-antibodies_1_75a69c.html

  Figure S2. Size distribution, canonical duplexes with 3’ 2-nt overhangs of virus derived small RNAs and viral genomic coverage depth of each nucleotide position by 21- to 23-nt vsiRNAs sequenced from brain (A) and hindlimb muscle (B) of Ifnar1-/-suckling mice infected with ZIKV at 4 dpi, either without (A, B) or with (C) co-immunoprecipitation by antibodies specific to AGOs (a repeat of ...

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• VWR

  Phallotoxins also stabilize F-actin, inhibiting depolymerization by cytochalasins, potassium iodide and elevated temperatures. Because the phalloidin conjugates are small, with an approximate diameter of 12–15 Å and molecular weight of

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