Oc gel
EPOXY AND SILICONE ADHESIVES
Temperature oC Gel Time Cure Time 25 (rm. temp) 5 hours 15 hours 50 1 hour 4 hours 75 10 minutes 30 minutes 100 5 minutes 10 minutes Transene Company Inc. 10 Electronics Avenue, Danvers MA 01923 Tel: 978-777-7860 Fax: 978-739-5640 www.transene.com. GENERAL PURPOSE EPOXIES. For Electronic Potting Applications
[DOCX File]Science
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Capacitance’ verses frequency plot of VHB 4910 at 25 oC. Figure S7. Dielectric constant and dielectric loss verses frequency plots of the 40% polymer content ion gel at -75 oC. Figure S8. Weight retention rate versus time plot of the ion gel storing at a high RH (85%). Figure S9.
[DOC File]labs.pbrc.edu
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10. Spin cells at 14000 rpm for 5 min at 4 oC to collect supernatant as the nuclear extract. 11. Measure protein concentration and adjust it to 1 μg/μl with the extraction buffer for use in. gel shift assay. (See the attached formula for each buffer). (Prepared by Jianping Ye, M.D.) 2 x gel shift reaction buffer
[DOC File]Tech-Dry
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Appearance: Pale green gel. Boiling point: (196 oC Vapour pressures (20 oC): Not allocated. Density (20 oC): approx 1.05 g/ml. Flash point: 92 oC. Flammability limits (%): LEL 0.9, UEL 8.0 (as to the main ingredient) Solubility in water: Dispersible. Other properties Autoignition point: 370 oC
[DOC File]Bezpečnostní list - SEPTODERM
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skladovat v originálních uzavřených obalech, odděleně od potravin a pitné vody; teplota skladování –20 až +25 oC (neskladovat na přímém slunečním světle). 8. Kontrola expozice a ochrana osob. 8.1. Technická opatření: dodržení podmínek manipulace a skladování; větrání, …
[DOC File]Molecular Biology – Final Laboratory Report
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Annealing temperatures of 56 oC and 59oC were used. PCR product was analyzed by gel electrophoresis, then amplified cDNA was extracted from the gel and cloned into the pENTR/D-TOPO plasmid (Figure 2). Escherichia coli cells were heat-shocked and transformed with the LAJ2- …
[DOC File]Agarose Gel Electrophoresis Protocol
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DNA Agarose Gel Electrophoresis. 1. Equilibrate a water bath at about 50 oC. 1. Make 400 ml of 1 X TAE buffer by diluting 10 ml TAE with 490 ml deionized water. 2. Measure 50 ml of 1 X TAE buffer and pour it into a 50 ml glass flask. 3. Weight out 0.7 grams of electrophoresis grade agarose and add it to 50 ml of 1 X TAE buffer solution in the ...
[DOCX File]ExpansionMicroscopy.org
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Submerge the gel, still on the slide, in 3 mL of freshly made digestion buffer (including proteinase K), then incubate for 3 hours at 60 °C. (Make sure to completely submerge the slice in the digestion buffer, and make sure it does not dry out by sealing the container with Parafilm.
[DOC File]General Guidelines for Preparing Samples for MS Analysis
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Store the gel bands/spots at 4 oC (if less than a day) or at -80 oC. Gel Visualization Methods. Use fresh solutions/buffers for all steps (or solutions/buffers dedicated to proteomics' samples). Wear a clean lab coat, gloves, hat and face mask when handling the gel (especially when excising the bands/spots).
[DOC File]Protein Purification- A Model Protocol
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Pick a single colony and make a 3 mL O/N culture, 37 oC –Transfer 2 mL of O/N into 1L (LB/KAN or AMP)-Grow to 0.4-0.6 OD600 (4-5 hrs)-Add IPTG to each liter Depends on your sample (0.2 mM Final Conc)-Shake at 30 C for 5 hrs-Pour Cultures into 1L bottles-Spin at 3.8 (3800) for 20 min
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