Protein molecular weight calculator
[DOCX File]community.asdlib.org
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The second section describes the use of electrospray ionization to determine the molecular weight of a protein using an ion trap mass analyzer. The third section shows how to identify an unknown protein using peptide mass fingerprinting with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF).
[DOC File]LABORATORY 5
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1. Lay your stained gel on the CM Rule & Relative Mobility Calculator (to be handed out in lab) with the tracking dye on the bottom line of the Rule (Relative Mobility = 1.0). Position the gel so the top of the molecular weight standard lane is aligned with the top line of the Rule (Relative Mobility = 0).
[DOC File]Gastroenterology - Stanford University
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Suspect if weight loss, malabsorptive diarrhea, arthritis, vague abdominal pain. Screen with antiendomysial IgA (most sensitive and specific) and/or antigliadin IgA and IgG. Alpha1-AT deficiency. If SPEP shows low alpha globulin levels, send for serum AT levels (
[DOC File]Fundamentals of Biochemistry
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Over two weeks you will purify a protein by gel filtration chromatography, determine its molecular weight and investigate the importance of protein structure to its function.-----LAB 3 – In this lab you will use electrophoresis to analyze the hemoglobin protein in normal and mutated forms.
[DOCX File]The c - Home | mBio
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™ G-25 Fine (GE Healthcare Life Sciences, Uppsala, Sweden), which yielded two major pools: high molecular weight polymeric materials and low molecular weight materials co-eluted with salts. High molecular weight material was next separated on an anion exchanger . HiPrep ™ 16/10 DEAE FF column (GE Healthcare Life Sciences) equilibrated with ...
[DOC File]BITC1311 Introduction to Biotechnology
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Apr 26, 2006 · The protein component of this phage consists of a protective coat that forms the tail assembly and the outer shell of the head. A single molecule of double-stranded DNA is located in the core of the phage head. The DNA molecule contains 48,502 base-pairs (molecular weight ≈ 3 x 107 g/mol) that code for approximately 50 different proteins.
[DOC File]bkemlaribflav - Southwestern University
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Your protein molecular weight standards should be placed in the boiling water bath at the same time as your samples, and 10 (L of the standards should be applied to the wells corresponding to the lanes for your standards. There are two sets of molecular weight standards that you should use in today's lab.
[DOC File]Cell Biology Workshop I - EvergreenStateCollege-Home
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Using several proteins as a data set, the average molecular weight of an amino acid in a protein is about 110 g/mole. The most common blood plasma protein in mammals is a globular protein called serum albumin. (It is usually about 60% of the total protein mass.)
[DOC File]Calculations
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1 nmole = molecular weight (ng) When to use the molecular weight: Molecular weights are used in calculations when you want to convert from concentrations expressed in Molar units (M, mM, M etc) to concentrations expressed in g/L, g/100 mL, % (w/v), mg/mL and vice versa. It is also used when you need to work out how much of a particular compound ...
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